教授华中农业大学生命科学技术学院
教师简介

    刘子铎,男 1954年12月生 博士 华中农业大学生命科学技术学院教授,博士生导师。
研究方向:微生物分子生物学
教学情况
1、主讲课程“基因操作原理”专业基础课2004年-2012年,4学时/周,共48学时,每年修课人数260-300人。
2、承担生物技术专业本科生课程设计和指导毕业论文。
科研情况
科研项目:
1、AT碱基定向诱变技术的建立和AHL-内酯酶的体外进化,国家自然科学基金,2004-2006
2、GC碱基特异替换技术的建立和AlbD酶的体外进化,国家自然科学基金,2006-2008
3、高频碱基替换技术的建立和gat 基因的体外定向进化,国家自然科学基金,2008-2010                                                                                       

4、新疆极端生境微生物灭生性除草剂抗性基因资源的挖掘,国家自然科学基金, 2012-2014
5、炭疽杆菌致死毒素的体外分子进化与疫苗及药物筛选,教育部科学技术研究重大项目2006—2009
6、新型转基因抗虫棉花材料创造与新品系培育,国家转基因重大专项,2009-2010
7、抗草甘膦除草剂基因的克隆和酶学性质分析,国家转基因重大专项,2008-2009
8、复合性状转基因抗虫水稻新品种的培育,国家转基因重大专项,2011-2012

近期发表学术论文:
1. Yang S,Li Q,Zeng Q,Zhang J,Yu Z,Liu Z,Conversion of Solid Organic Wastes into Oil via Boettcherisca peregrine (Diptera: Sarcophagidae) Larvae and Optimization of Parameters for Biodiesel Production. PLoS One. 2012;7(9):e45940. doi: 10.1371
2. Huang W,Lin Y,Yi S,Liu P,Shen J,Shao Z,Liu Z. QsdH, a Novel AHL Lactonase in the RND-Type Inner Membrane of Marine Pseudoalteromonas byunsanensis Strain 1A01261. PLoS One. 2012; 7(10):e46587. doi: 10.1371
3. Dong F,Shi R,Zhang S,Zhan T,Wu G,Shen J,Liu Z,Fusing the vegetative insecticidal protein Vip3Aa7 and the N terminus of Cry9Ca improves toxicity against Plutella xylostella larvae. Appl Microbiol Biotechnol. 2012 Nov; 96(4):921-9.
4. Liu P,Gao Y,Huang W,Shao Z,Shi J,Liu Z. A novel bioassay for high-throughput screening microorganisms with N-acyl homoserine lactone degrading activity,Appl Biochem Biotechnol. 2012,167(1):73-80.
5. Wu G,Feng C,Cao S,Guo A,Liu Z. Identification of New Dominant-Negative Mutants of Anthrax Protective Antigen Using Directed Evolution,Appl Biochem Biotechnol.2012 Sep 5. 6.
6. Dong F,Zhang S,Shi R,Yi S,Xu F,Liu Z,Ser-Substituted Mutations of Cys Residues in Bacillus thuringiensis Vip3Aa7 Exert a Negative Effect on its Insecticidal Activity, Curr Microbiol. 2012 Nov;65(5):583-8.
7. Huang X,Zhao Y,Dai Y,Wu G,Shao Z,Zeng Q,Liu Z. Cloning and biochemical characterization of a glucosidase from a marine bacterium Aeromonas sp. HC11e-3,World J Microbiol Biotechnol. 2012; 28(12):3337-44.
8. Gaobing Wu, Yuzhi Hong, Aizhen Guo, Chunfang Feng, Sha Cao, Cheng-Cai Zhang, Ruiping Shi, Yadi Tan, and Ziduo Liu*.  A chimeric protein that functions both as an anthrax dual-target antitoxin and trivalent vaccine. Antimicrob. Agents Chemother  2010, 54:4750-4757.
9. Gaobing Wu, Chunfang Feng, Yuzhi Hong , Aizhen Guo, Sha Cao, Junli Dong, Ling Lin, Ziduo Liu*. Soluble expression and purification of the protective antigen in E. coli and identification of a novel dominant-negative mutant N435C. Applied Microbiology and Biotechnology 2010, 87:609-616.
10. Xiaoyu Fu, Pengfu Liu, Ling Lin, Yuzhi Hong, Xiaoluo Huang, Xin Meng,  Ziduo Liu*. A cold-active endoglucanase (Cel9P) from a sea bacteria Paenibacillus provencensis BME-14, Applied biotechnology and biochemistry  2010,160: 1627-36
11. Xiaoluo Huang, Zongze Shao, Yuzhi Hong, Ling Lin, Chanjuan Li, Fei Huang, Hui Wang, Ziduo Liu*. Cel8H, a novel endoglucanase from the halophilic bacterium Halomonas sp. strain S664: molecular cloning, heterogonous expression and biochemical characterization, The Journal of Microbiology 2010, 48(3)318-324.
12. Junli Dong, Yuzhi Hong, Zongze Shao, Ziduo Liu*.  Molecular cloning, purification and properties of a novel acidic, pH-stable endoglucanase (Cel5D) from Martelella mediterranea. The Journal of Microbiology. 2010, 48:393-398
13. Shuilian Chen, Yuzhi Hong, Zongze Shao, Ziduo Liu* .  A cold-active β-glucosidase (Bgl1C) from a sea bacteria Exiguobacterium oxidotolerans A011, World Journal of Microbiology and Biotechnology. 2010, 26:1427-1435.
14. Jing Wang, Zongze Shao, Yuzhi Hong, Chanjuan Li, Xiaoyu Fu, Ziduo Liu*. A Novel β-mannanase from Pantoea agglomerans A021: Gene Cloning, Expression, Purification and Characterization. World Journal of Microbiology and Biotechnology. 2010 , 26:1777-1785
15. Xiaoxia Mao, Yuzhi Hong, Zongze Shao, Yan Zhao, Ziduo Liu* .  A novel cold active and alkali-stable β-glucosidase gene isolated from the marine bacterium Martelella mediterranea, Applied biotechnology and biochemistry.  2010, 162:2136-2144
16. Pengfu Liu, Yuzhi Hong, Yongjun Lin, Xiaoyu Fu, Ling Lin , Congcong Li, Xin Meng,  Ziduo Liu*.  A frequency-controlled random mutagenesis method for GC-rich gene. Analytical Biochemistry 2009, 388: 356-358
17. Ling Lin, Xin Meng, Pengfu Liu, Yuzhi Hong, Gaobing Wu, Xiaoluo Huang, Congcong Li, Junli Dong, Liang  Xiao, Ziduo Liu*.  Improved Catalytic Efficiency of Endo-β-1, 4-glucanase from Bacillus subtilis BME-15 by Directed Evolution. Applied Microbiology and Biotechnology 2009, 82: 671-679
18. Xin Meng, Zongze Shao, Yuzhi Hong, Ling Lin, Chanjuan Li, Ziduo Liu*.  A novel pH-stable xylanase from deep-sea Cellulomonas fermentans JK4. Jounary of  Microbiology Biotechnology 2009,19: 1077-1084
19. Chanjuan Li, Yuzhi Hong, Zongze Shao, Ling Lin , Xiaoluo Huang, Pengfu Liu, Gaobing Wu, Xin Meng, Ziduo Liu*. A novel alkali-stable, cellulase-free xylanase fromdeep-sea Kocuria sp. Mn22, Journal Microbiology Biotechnology  2009 , 19: 873-880